ValidPrime Validprime kit:ValidPrime® Rat – Probe assay – 250 rxns The blue color of the

The blue color of the mix helps visualize pipetting in clear or white plates and does not interfere with the stability of the product or qPCR efficiency

The number of reactions is based on 20μl-qPCR reactions

The assay has very high PCR efficiency (E > 90% in tested commercial master mixes) and produces negligible amount of primer dimer products

Normalization should include a reference gene

ValidPrime® is highly optimised and specific to a non-transcribed locus of gDNA that is present in exactly one copy per haploid normal genome

ValidPrime Validprime kit:ValidPrime® Rat – Probe assay – 250 rxns The blue color of theValidPrime replaces the need to perform no reverse transcriptase (RT( )) controls to test for the presence of genomic DNA (gDNA) for all samples in your real time quantitative PCR (qPCR) profiling. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT( ) reactions plus m x n qPCR controls. When using ValidPrime only m + n + 1 controls are needed. ValidPrime will minimize the amount of control reactions