Proteinase K Powder（RNase-Free, DNase-Free） _ 10401ES DNA&RNA Extraction A: You can first mix

A: You can first mix the large sample

Purification Ni column affinity purification Reaction Condition The reaction buffer should be incubated at 37℃ -70℃ for 1h

40619-E***

2 μm filtered concentrated solution in 35% Acetonitrile and 0

[4] Wang Y

Proteinase K Powder（RNase-Free, DNase-Free） _ 10401ES DNA&RNA Extraction A: You can first mixProteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29. 3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common